Emploi-type : Ingénieur d’études en biochimie / Ingénierie des protéines
BAP : A
Missions : Le candidat sélectionné (H/F) participera aux travaux de l’équipe et en particulier à un projet financé par l’ANR qui vise à exprimer des protéines recombinantes pour développer un vaccin contre le paludisme.
Activités principales
• Clonage de gènes dans des vecteurs d’expression
• Culture cellulaire (Systèmes Eucaryote et procaryote)
• Transfection de cellules
• Expression et purification de protéines recombinantes
• Culture de Plasmodium
Activités associées
• Gérer les stocks des réactifs
• Participer aux tâches communes du laboratoire (entretien, commandes, …)
Connaissances
• Connaissances générales en techniques de bases d’analyses biochimiques, cellulaires et moléculaires
• Bonnes connaissances de tests biochimiques et biologiques quantitatifs (ELISA,
immunofluorescence, etc.),
• Bonnes connaissances théoriques et pratiques des techniques de cytométrie en flux
(caractérisation et phénotypage cellulaire)
• Bonnes notions d’Anglais : compréhension suffisante pour suivre la littérature scientifique
spécialisée, comprendre les fiches techniques et les protocoles
• Maitrise des analyses statistiques de base
• Maitrise des outils informatiques, Word, Excel, Powerpoint, logiciels statistiques
(GraphPadPrism), logiciels d’analyse d’images (image J).
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